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Spatial and Temporal Expression of P-Glycoprotein in the Congenitally Hydrocephalic HTX Rat Brain

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Spatial and Temporal Expression of P-Glycoprotein in the Congenitally Hydrocephalic HTX Rat Brain

Auteurs : S. Kuwahara [Japon] ; Y. Sada [Japon] ; T. Moriki [Japon] ; T. Yamane [Japon] ; H. Hara [Japon]

Source :

Pathology - Research and Practice [ 0344-0338 ] ; 1996.

RBID : ISTEX:FE4578E6818B0EE20FA484ADD81D5AC689B15E66

English descriptors

Abstract

Summary: The purpose of the present study is to examine spatial and temporal expression of P-glycoprotein in the brain of congenitally hydrocephalic HTX rats. P-glycoprotein has been reported not only as a drug efflux pump but also one of the factors that restricts brain edema. We examined the rat brain from postnatal day 1 to 60 using light and electron microscopy, immunohistochemistry, Western immunoblot and reverse transcriptase-polymerase chain reaction (RT-PCR) methods with monoclonal antibody specific for P-glycoprotein.Immunohistochemically, the positive anti-P-glycoprotein reactivity was found in capillaries of the normal control rat cerebrum. In the hydrocephalic HTX rat brains, it was also found in the capillaries, but only very weak to no reactivity was found in the capillaries of the spongy changes and cystic wall in the subcortical and lateral periventricular white matter. Immunoelectron microscopically, the reaction product was found exclusively on the luminal surface of the capillary endothelium in control rats. A tracer study with intracardiac perfusion of lanthanum chloride showed that lanthanum penetrated the tight junctions and passed through the intercellular space.In the Western immunoblot analysis, P-glycoprotein of 170 kDa was detected clearly in most normal control rat brains but it was not found in the hydrocephalic HTX rat brains. Moreover, mdr1 P-glycoprotein gene expression in the subcortical white matter was examined by RT-PCR. It was detected in all normal control rat brains, but not found in the hydrocephalic HTX rat brains. The results suggested that the absence of P-glycoprotein expression in the capillaries of deep subcortical and lateral periventricular white matter of hydrocephalic HTX rats led to a deficiency of the blood-brain barrier and might be related to vasogenic edema and to the formation of the spongy changes and cystic cavities.

Url:

https://api.istex.fr/ark:/67375/6H6-RCF389BD-M/fulltext.pdf

DOI: 10.1016/S0344-0338(96)80018-5

Affiliations:

Japon

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<author><name sortKey="Moriki, T" sort="Moriki, T" uniqKey="Moriki T" first="T." last="Moriki">T. Moriki</name>
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<author><name sortKey="Yamane, T" sort="Yamane, T" uniqKey="Yamane T" first="T." last="Yamane">T. Yamane</name>
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<term>Brain edema</term>
<term>Congenital hydrocephalus</term>
<term>HTX rat brain</term>
<term>P-glycoprotein</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Acta</term>
<term>Acta neuropathol</term>
<term>Amersham life science</term>
<term>Bacterial transport proteins</term>
<term>Basal ganglia</term>
<term>Basement membrane</term>
<term>Biochim biophys acta</term>
<term>Blood flow</term>
<term>Brain barrier</term>
<term>Brain capillaries</term>
<term>Brain capillary</term>
<term>Brain edema</term>
<term>Brain tissue</term>
<term>Bram capillanes</term>
<term>Capillary</term>
<term>Cdna equivalent</term>
<term>Cerebral cortex</term>
<term>Cerebral edema</term>
<term>Cerebrum</term>
<term>Clea japan</term>
<term>Congenital</term>
<term>Congenital hydrocephalus</term>
<term>Congenitally hydrocephalic</term>
<term>Control rats</term>
<term>Cystic</term>
<term>Cystic cavities</term>
<term>Cystic lesion</term>
<term>Cystic wall</term>
<term>Deep subcortical</term>
<term>Drug efflux</term>
<term>Edema</term>
<term>Edema constituents</term>
<term>Edema fluid</term>
<term>Electron micrograph</term>
<term>Electron microscopically</term>
<term>Endothelial</term>
<term>Endothelial cell</term>
<term>Endothelial cells</term>
<term>Entire length</term>
<term>Ependymal cell layer</term>
<term>Ethanol series</term>
<term>Ether anesthesia</term>
<term>Ethidium bromide</term>
<term>Experimental hydrocephalus</term>
<term>Extracellular space</term>
<term>Foreign agents</term>
<term>Glial cells</term>
<term>Hematoxylin counterstain</term>
<term>House mouse</term>
<term>Hydrocephalic</term>
<term>Hydrocephalic brain</term>
<term>Hydrocephalic rats</term>
<term>Hydrocephalus</term>
<term>Immunoelectron microscopically</term>
<term>Immunohistochemical expression</term>
<term>Immunohistochemical reactivity</term>
<term>Initial change</term>
<term>Intercellular space</term>
<term>Intercellular spaces</term>
<term>Interstitial edema</term>
<term>Intraventricular injection</term>
<term>Intraventricular pressure</term>
<term>Ionic lanthanum</term>
<term>Kochi</term>
<term>Kuwahara</term>
<term>Lanthanum</term>
<term>Lanthanum chloride</term>
<term>Lateral</term>
<term>Lateral periventricular</term>
<term>Lateral ventricle</term>
<term>Lateral ventricles</term>
<term>Lesion</term>
<term>Luminal</term>
<term>Luminal surface</term>
<term>Mdel gene expression</term>
<term>Mdrl gene expression</term>
<term>Milk powder</term>
<term>Molecular weight</term>
<term>Monoclonal</term>
<term>Monoclonal antibody</term>
<term>Morphological study</term>
<term>Neuropathol</term>
<term>Normal control</term>
<term>Normal control rats</term>
<term>Normal rats</term>
<term>Obstructive hydrocephalus</term>
<term>Oncogene science</term>
<term>Osmotic pressure</term>
<term>Other hand</term>
<term>Paracellular pathway</term>
<term>Periventricular</term>
<term>Periventricular area</term>
<term>Positive reaction</term>
<term>Postnatal</term>
<term>Present study</term>
<term>Previous study</term>
<term>Primer</term>
<term>Protein sample</term>
<term>Rat</term>
<term>Rats immunostained</term>
<term>Reaction product</term>
<term>Room temperature</term>
<term>Specific primers</term>
<term>Spongy</term>
<term>Spongy change</term>
<term>Spongy changes</term>
<term>Subcortical</term>
<term>Subependymal area</term>
<term>Temporal expression</term>
<term>Tight junctions</term>
<term>Tracer</term>
<term>Tracer study</term>
<term>Vasogenic</term>
<term>Vasogenic edema</term>
<term>Ventricle</term>
<term>Ventricular dilatation</term>
<term>Weak immunoreaction</term>
<term>Weak reaction</term>
<term>Western immunoblot</term>
<term>Western immunoblot analysis</term>
<term>White matter</term>
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<front><div type="abstract" xml:lang="en">Summary: The purpose of the present study is to examine spatial and temporal expression of P-glycoprotein in the brain of congenitally hydrocephalic HTX rats. P-glycoprotein has been reported not only as a drug efflux pump but also one of the factors that restricts brain edema. We examined the rat brain from postnatal day 1 to 60 using light and electron microscopy, immunohistochemistry, Western immunoblot and reverse transcriptase-polymerase chain reaction (RT-PCR) methods with monoclonal antibody specific for P-glycoprotein.Immunohistochemically, the positive anti-P-glycoprotein reactivity was found in capillaries of the normal control rat cerebrum. In the hydrocephalic HTX rat brains, it was also found in the capillaries, but only very weak to no reactivity was found in the capillaries of the spongy changes and cystic wall in the subcortical and lateral periventricular white matter. Immunoelectron microscopically, the reaction product was found exclusively on the luminal surface of the capillary endothelium in control rats. A tracer study with intracardiac perfusion of lanthanum chloride showed that lanthanum penetrated the tight junctions and passed through the intercellular space.In the Western immunoblot analysis, P-glycoprotein of 170 kDa was detected clearly in most normal control rat brains but it was not found in the hydrocephalic HTX rat brains. Moreover, mdr1 P-glycoprotein gene expression in the subcortical white matter was examined by RT-PCR. It was detected in all normal control rat brains, but not found in the hydrocephalic HTX rat brains. The results suggested that the absence of P-glycoprotein expression in the capillaries of deep subcortical and lateral periventricular white matter of hydrocephalic HTX rats led to a deficiency of the blood-brain barrier and might be related to vasogenic edema and to the formation of the spongy changes and cystic cavities.</div>
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Spatial and Temporal Expression of P-Glycoprotein in the Congenitally Hydrocephalic HTX Rat Brain

Spatial and Temporal Expression of P-Glycoprotein in the Congenitally Hydrocephalic HTX Rat Brain

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Abstract

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